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Research demonstrates that copper-binding peptides including GHK-Cu suppress the NF-κB pathway through direct molecular interactions, with nuclear translocation of NF-κB p65 decreased by approximately 30% upon treatment and molecular modeling showing specific peptide sequences exhibit high binding affinity to NF-κB p65 (up to -8.8 kcal/mol)

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Analyses were performed in R (version 4.5.1) using the gemtc and BUGSnet packages, with four parallel Markov Chain Monte Carlo chains of 50,000 iterations each, including a 20,000-iteration burn-in period