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After 24 h, half the supernatant was removed and replaced with 100 µl of fresh medium containing ± OligoG (8%) and nystatin (4 µg/ml) (final concentration of 4% OligoG and 2 µg/ml nystatin) with a further 24 h incubation (20 rpm rocking, 37°C

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"When supplementing with glutathione to correct glutathione deficiency or maintain optimal levels, supplement daily." How Much Glutathione Should You Take Daily