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Oxidative stress is reinforced by several reactive species, including H 2 O 2 , singlet oxygen, other radicals, and non-radicals, which are consistently produced in the body due to the aerobic metabolism, and thereby potentially altering basic structural components such as proteins, lipids, and nucleic acids (Weidinger and Kozlov, 2015)

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Sodium dodecyl sulphate (SDS) contains both hydrophobic and strong ionogenic groups and binding to hydrophobic regions results in a layering of negative charges on the protein’s surface, causing irreversible unfolding of the structure