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For H3K36me3 and keratin 14, antigen retrieval was performed with BOND Epitope Retrieval 1 (AR9961, Leica) and for vit B 12 with BOND Epitope Retrieval Solution 2 (Leica Biosystems, AR9640) for 20 min, whereas for Ki67, sections were dewaxed as part of the antigen retrieval process using the low pH EnVision FLEX Target Retrieval Solutions (Dako) for 20 min at 97 °C using a PT Link (Dako-Agilent)

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Cagrilintide is a synthetic long-acting amylin analogue peptide designed to mimic and enhance the effects of natural amylin, a hormone co-secreted with insulin from pancreatic beta cells